Performance
The PAXgene Blood DNA Tubes simplify the collection process of whole blood for subsequent DNA purification by enabling collection, immediate stabilization, transportation, and storage of blood samples in the same tube. DNA purified from specimens in PAXgene Blood DNA Tubes using the PAXgene Blood DNA Kit is of high quality and high molecular weight, attesting to the superior stabilization afforded (Figure 1).
Principle
The PAXgene Blood DNA Tubes are closed, evaluated collection tubes containing a proprietary blend of reagents that both prevents blood coagulation and stabilizes genomic DNA in white blood cells. Because this stabilization is afforded at several temperatures, the PAXgene Blood DNA Tubes also serve as vessels for the storage and transportation of blood samples, and stabilization is robust to variation in handling.
Procedure
Blood samples are collected using standard phlebotomy practice directly into PAXgene Blood DNA Tubes (Figure 2). Samples can then be immediately processed, stored, or shipped within the same tube. Because the PAXgene Blood DNA Tubes are carefully calibrated, the tube draws the 8.5 mL blood needed to maintain the correct additive to blood ratio (0.24) for optimal stabilization.
Applications
The DNA purified from whole blood collected and stored in PAXgene Blood DNA Tubes can be used in a wide range of downstream applications, including:
- PCR, multiplex, long-range, and quantitative, real-time PCR
- Southern blotting
- Pharmacogenomic studies
- SNP discovery and SNP genotyping
- Next-generation sequencing
![Genomic DNA isolated from 8 blood donors using the PAXgene Blood DNA System. [A] Agarose gel analysis; [B] pulsed-field gel electrophoresis for enhanced separation of high-molecular-weight genomic DNA. M: markers.](/storage/images/_processed_/9/7/csm_High_quality_and_high_molecular_weight_of_genomic_DNA_03f21d3802.jpg "Figure 1. High quality and high molecular weight of genomic DNA.")
