Resources dedicated to cfDNA research

Circulating cell-free DNA (ccfDNA)

An important biomarker for non-invasive prenatal testing (NIPT) and liquid biopsy, circulating cell-free DNA (ccfDNA) is easily obtainable through a simple blood draw. ccfDNA from plasma has the ability to provide a breadth of information to researchers.

Did you know?

  • ccfDNA is present in low quantity in blood
  • ccfDNA can be further diluted by genomic DNA (gDNA) from apoptotic or lysed blood cells
  • The type of anticoagulant and crosslinking reagents in blood collection tubes can impact the quality of ccfDNA

Standardize your workflow with the PAXgene Blood ccfDNA System (RUO)*
to preserve the ccfDNA profile in your blood sample


Blood collection, stabilization and ccfDNA extraction for reliable downstream assay performance

Collecting specimens in the PAXgene Blood ccfDNA Tube

Stabilization chemistry matters

Learn about the non-crosslinking stabilization chemistry in the PAXgene Blood ccfDNA Tube and how other tubes may impact analytical sensitivity in downstream reactions.

Understand the importance of preanalytical sample preparation/stabilization and how it may affect analytical sensitivity in downstream analysis:

Stabilization chemistry matters

Multimodal Liquid Biopsy Analysis from blood samples collected in PAXgene Blood ccfDNA Tubes

Check out our poster presented at the AACR Virtual Meeting II

Explore ccfDNA, CTCs and gDNA from the same sample

Five minute read on how to perform multimodal analysis from a single blood sample. Learn how to extract and analyze ccfDNA and additional analytes like RNA from CTCs or gDNA from a single sample of blood collected in the PAXgene Blood ccfDNA Tube*.

Trusted quality samples with PreAnalytiX

Learn more about optimal quantification methods, reliable stability claims and long shelf life of the PAXgene Blood ccfDNA Tube* which is backed by robust data.  

Research Applications

Non-invasive prenatal testing (NIPT)

Researchers in the field of non-invasive prenatal testing analyze fetal DNA present among maternal ccfDNA from blood of a pregnant woman to determine the risk that the fetus will be born with certain genetic abnormalities.

Liquid Biopsy

Liquid biopsy is a minimally-invasive method of collecting a patient biofluid sample to test for biomarkers of disease. Liquid biopsy is commonly used for many applications in cancer research.

Click here to learn more about QIAGEN's manual and automated Sample to Insight workflows for liquid biopsy and cancer research applications.

*The PAXgene Blood ccfDNA Tube is for Research USE Only. Not for use diagnostic procedure.


Meet our experts on upcoming events to discuss the possibilities how to optimize your preanalytical workflows

Mark your calendar

Learn more
Man listening to a webinar

Watch this recent PreAnalytiX webinar focusing on "Liquid Biopsy Workflow Optimization for Improved Assay Sensitivity and Multianalyte Applications of Cell-Free RNA, DNA and CTCs"

Get more insight

Learn more

Discover our variety of scientific posters which were presented at conferences around the world

Explore the details

Learn more

Service

Contact us for troubleshooting and support

Global Contacts

Technical Service

Customer Care