The PAXgene Blood RNA System consolidates and integrates the key steps of whole blood collection, intracellular RNA profile stabilization and RNA purification. By minimizing the unpredictability associated with RNA processing, the system generates high yields of high-quality intracellular RNA for accurate RNA analysis.
The PAXgene Blood RNA System consists of the PAXgene Blood RNA Tube and different purification kits that accommodate your throughput and automation needs. RNA purified from blood using this system is optimal for a range of downstream applications, including RT-PCR and quantitative real-time RT-PCR.
Upon blood specimen collection, ex vivo gene induction and downregulation, initiation of cell death programs, and enzymatic activity can all contribute to changes in cellular RNA transcript levels. The patented additive in PAXgene Blood RNA Tubes lyses cells in the collected blood, which effectively inhibits cellular processes that can lead to altered RNA levels. Additionally, RNA molecules are protected against enzymatic degradation. The result is a stabilized snapshot of the gene expression profile at the time of blood collection.
The proprietary reagent in PAXgene Blood RNA Tubes stabilizes intracellular RNA in collected blood specimens for 3 days at room temperature (15–25°C) and 5 days at 2–8°C. Thus, temperature fluctuations up to 25°C during transportation and storage do not impact the intracellular RNA profile and the integrity of the RNA that is subsequently purified. Furthermore, procedural variation is eliminated because collection, stabilization, transport and storage are all done in a single tube.
Differences in preanalytical methods generate variability in RNA quality and yield, which can render results from different labs and between different operators incomparable. The PAXgene Blood RNA System standardizes procedures with convenient BD Vacutainer technology, which enhances patient and healthcare worker safety, provides sample protection and facilitates consistent blood draw volumes. Furthermore, the straightforward purification workflow enables uniformity of handling to reduce user-to-user variability.
Chemistry of the PAXgene Blood RNA Kit systematically digests proteins, filters out cellular debris and depletes genomic DNA through a DNase treatment step prior to eluting molecules bound to a silica membrane. The resulting eluate contains high-quality RNA with minimum carryover contaminants that can confound downstream analyses.