April 2022

In this study, the scientists describe how they tried to identify a feasible method to characterize the tissue-associated microbiome of esophageal adenocarcinoma (EAC) biopsies, and to investigate differences in the microbiome of saliva, esophageal tissue, and fecal samples by disease state. PAXgene Tissue Fixation was established as a feasible method for microbiome analysis and utilized to identify a distinct microbial shift in tissue biopsies from patients with EAC.

Analysis of Fecal, Salivary, and Tissue Microbiome in Barrett's Esophagus, Dysplasia, and Esophageal Adenocarcinoma

Nikole Radani^1,*, Amira Metwaly^2,*, Sandra Reitmeier³, Theresa Baumeister¹, Jonas Ingermann¹, Julia Horstmann¹, Akanksha Anand¹, Ingrid Gatz⁴, Florian Kohlmayer⁴, Klaus-Peter Janssen^5,6, Julia Slotta-Huspenina^6,7, Roland M. Schmid¹, Dirk Haller², Julian A. Abrams⁸, and Michael Quante^1,9

_{1 Department of Internal Medicine, Technical University of Munich, Munich, Germany; 2 Chair of Nutrition and Immunology, Technical University of Munich, Munich, Germany; 3 ZIEL-Institute for Food and Health, Technical University of Munich, Munich, Germany; 4 Institute of Medical Informatics, Statistics and Epidemiology, University Hospital rechts der Isar, TUM, Munich, Germany; 5 Department of Surgery, Klinikum rechts der Isar, TU München, Munich, Germany; 6 The Biobank of Klinikum rechts der Isar/Technical University Munich (MTBIO), Munich, Germany; 7 Institute of Pathology, Technical University of Munich, Munich, Germany; 8 Department of Medicine, Columbia University Irving Medical Center, New York, New York; and 9 Department of Internal Medicine II, University of Freiburg, Freiburg, Germany}

Gastro Hep Advances 1:755–766 (2022). https://doi.org/10.1016/j.gastha.2022.04.003