Fixation & Stabilization
The PAXgene Tissue Systems are formalin-free solutions that enable the integrated fixation, storage, and processing of tissue samples for both histological and molecular analysis of the same tissue. The systems consist of a fixation reagent, a stabilization reagent, and kits for the purification of DNA (PAXgene Tissue DNA Kit), RNA (PAXgene Tissue RNA Kit), or total RNA including microRNA or miRNA (PAXgene Tissue miRNA Kit). Additionally, supplementary protocols for other applications, including purification of full-length proteins, are available (see Qproteome FFPE Tissue Kit).
Rapid and complete fixation without inhibiting downstream applications
The fixation reagent contained in the PAXgene Tissue Container and PAXgene Tissue FIX Container rapidly penetrates and fixes tissue with a fixation rate of approximately 1 mm per 30 minutes.* Morphology and biomolecules are preserved without destructive cross-linking and degradation found in formalin-fixed tissues, and no molecular modifications are introduced that can inhibit sensitive downstream applications such as quantitative PCR or RT-PCR.
Straightforward stabilization for preservation of biomolecules
After fixation in PAXgene Tissue FIX, tissues are transferred into the PAXgene Tissue STABILIZER to stop the fixation process and optimally protect biomolecules for long-term storage. Tissues fixed and stabilized using PAXgene Tissue Fixation & Stabilization Systems are crosslinking-free and have been repeatedly demonstrated to contain biomolecules of high quality and integrity for sensitive molecular analyses.
Exceptional stabilization for storage at different temperatures
Stored in PAXgene Tissue STABILIZER, tissues can be kept for weeks at room temperature (15–25°C) or refrigerated (2–8°C), and for several years when stored at –20°C to –80°C, without negative effects on the morphology of the tissue or the integrity of its nucleic acids.
Compatible with standard tissue sectioning and staining procedures
Tissues fixed and stablized with PAXgene Tissue FIX and PAXgene Tissue STABILIZER can be embedded in paraffin using standard procedures. The resulting PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue samples can be sectioned for histological studies in the same way as formalin-fixed, paraffin-embedded (FFPE) tissues, requiring no specialized equipment or additional protocol steps. Standard staining protocols using hematoxylin/eosin or immunohistochemistry (IHC) generate slides with high staining intensity and clearly visible cellular features for histological assessment and tissue pathology evaluation. For examples of different tissue types from diverse species and using different stains, please visit the Tissue Atlas. Furthermore, PFPE tissue samples enable a multi-modal approach to tissue pathology analysis because high-quality nucleic acids and proteins can be extracted from the same sample used for histological assessment.
* Fixation rates depend on type and size of tissue.