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PAXgene Tissue Systems

The problem

Simultaneous preservation of biomolecules, such as nucleic acids and proteins, and histomorphology features of a tissue sample is challenging. Snap-freezing in liquid nitrogen (N2) is the preferred method for biomolecule preservation, but the procedure damages tissue morphology. Furthermore, sectioning unfixed, frozen samples is technically demanding and requires specialized equipment. Formalin has been used for decades to preserve tissue histomophology, but formalin cross-links proteins and nucleic acids, making extraction of high-quality biomarkers from formalin-fixed, paraffin-embedded tissue difficult or impossible.

The Solution

The PAXgene Tissue Systems are formalin-free solutions for the simultaneous preservation of histomorphology and biomolecules and the purification of high-quality RNA, DNA, miRNA, proteins, and phosphoproteins from the same sample. Tissue specimens are collected, fixed, and stabilized with the PAXgene Tissue fixation and stabilization products. PAXgene Tissue fixed tissue can be processed and embedded in paraffin similarly to formalin-fixed tissue, and biomolecules are extracted using a series of companion kits: the PAXgene Tissue DNA System for DNA purification, the PAXgene Tissue RNA System for RNA purification, or the PAXgene Tissue miRNA System for purification of RNA including miRNA. Additionally, supplementary protocols are available for purification of full-length proteins from PAXgene Tissue fixed, paraffin-embedded tissue with the Qproteome FFPE Tissue Kit.

 

Features and benefits

Simultaneous preservation of histomorphology and biomolecules. The same sample can be used for standard histomorphological and molecular analyses of nucleic acids and proteins.

 

Rapid and convenient tissue fixation. Tissue samples are fixed at room temperature (15–25°C) in as little as 2 hours (depending on tissue type and size) and can be embedded and processed in paraffin.

 

Convenient tissue storage options. Tissue remains stable for days at room temperature, for weeks when refrigerated (2–8°C), and for years when frozen (–20°C or –80°C).

 

Multi-modal analysis of samples. PAXgene Tissue fixed (PF) samples as well as PAXgene Tissue fixed, paraffin-embedded (PFPE) tissue samples are suitable for a range of analyses, including histochemical staining, immunohistochemistry, in situ hybridization, gene expression analysis, genetic analysis, sequencing, protein and phosphoprotein analysis.

 

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