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PAXgene Blood RNA Tube (IVD)

For collection of whole blood samples for molecular diagnostic testing

  • Coupled with the PAXgene Blood RNA Kit (IVD), constitutes the FDA-cleared, CE-marked PAXgene Blood RNA System
  • Single tube for collection, transport, and storage
  • Immediate stabilization of intracellular RNA at collection
  • Specimen transport and storage at room or refrigerated temperatures
  • Frozen specimen storage for up to eight years
  • Standardized preanalytic processing of samples

 

Feature Specification
Tube size 16 x 100 mm
Blood draw volume  2.5 ml
Additive/concentration Additive 6.9 ml
Length of stabilization up to 3 days at room temperature (15–25°C)
up to 5 days at 2–8°C
8 years at –20°C or –70°C*
Closure type/color BD Hemogard/Red
Label type Paper
Quantity 100/box

*Long-term study of blood storage in PAXgene Blood RNA Tubes is ongoing.

 

Intended use

The PAXgene Blood RNA System consists of a blood collection tube (PAXgene Blood RNA Tube) and a nucleic acid purification kit (PAXgene Blood RNA Kit). It is intended for the collection, transport, and storage of blood and stabilization of intracellular RNA in a closed tube and subsequent isolation and purification of intracellular RNA from whole blood for RT-PCR used in molecular diagnostic testing.

Performance characteristics of the PAXgene Blood RNA System have been established with FOS and IL1B gene transcripts. The user is responsible for establishing appropriate PAXgene Blood RNA System performance characteristics for other target transcripts.

Relative transcript level changes of IL1B in blood stored in situ at –20°C in PAXgene Blood RNA Tubes.
Relative transcript level changes of FOS in blood stored in situ at –20°C in PAXgene Blood RNA Tubes.
Blood collected in duplicate from each of ten subjects was stored at –20°C. At each indicated time point, RNA was purified from duplicate tubes with the PAXgene Blood RNA Kit and analyzed using a [...]
Relative transcript level changes of IL1B in blood stored in situ at –20°C in PAXgene Blood RNA Tubes.
Relative transcript level changes of IL1B in blood stored in situ at –20°C in PAXgene Blood RNA Tubes.
Blood collected in duplicate from each of ten subjects was stored at –20°C. At each indicated time point, RNA was purified from duplicate tubes with the PAXgene Blood RNA Kit and analyzed using a [...]
Relative transcript level changes of IL1B in blood stored in situ at –20°C in PAXgene Blood RNA Tubes.
Integrity of RNA purified from blood stored in situ in PAXgene Blood RNA Tubes at –20°C.
Mean RNA integrity number (RIN) scores for duplicate specimens (subjects 1–10) and for all specimens in total (mean) are shown for the indicated storage times. The error bars indicate the upper and [...]
Relative transcript level changes of FOS in blood stored in situ at –70°C in PAXgene Blood RNA Tubes.
Relative transcript level changes of FOS in blood stored in situ at –70°C in PAXgene Blood RNA Tubes.
Blood collected in duplicate from each of ten subjects was stored at –70°C. At each time point indicated, RNA was purified from duplicate tubes with the PAXgene Blood RNA Kit and analyzed using a [...]
Relative transcript level changes of IL1B in blood stored in situ at –70°C in PAXgene Blood RNA Tubes.
Relative transcript level changes of IL1B in blood stored in situ at –70°C in PAXgene Blood RNA Tubes.
Blood collected in duplicate from each of ten subjects was stored at –70°C. At each time point indicated, RNA was purified from duplicate tubes with the PAXgene Blood RNA Kit and analyzed using a [...]
Integrity of RNA purified from blood stored in situ in PAXgene Blood RNA Tubes at –70°C.
Integrity of RNA purified from blood stored in situ in PAXgene Blood RNA Tubes at –70°C.
Mean RNA integrity number (RIN) scores for duplicate specimens (subjects 1–10) and for all specimens in total (mean) are shown for the indicated storage times. The error bars indicate the upper and [...]
Reproducibility between kit lots.
Reproducibility between kit lots.
Blood samples from 30 different donors were collected in PAXgene Blood RNA Tubes (12 tubes per donor, 360 tubes in total). The contents of the tubes from 3 donors were pooled and subsequently [...]
Reproducibility between users.
Reproducibility between users.
Blood samples from 30 different donors were collected in PAXgene Blood RNA Tubes (12 tubes per donor, 360 tubes in total). The contents of the tubes from 3 donors were pooled and subsequently [...]
RNA stability at 2–8°C: FOS.
RNA stability at 2–8°C: FOS.
Blood was drawn from 10 donors, with duplicate samples, and stored at 2–8°C for the indicated number of days, followed by total RNA purification. [A] Blood was collected and stored in PAXgene [...]
RNA stability at 2–8°C: IL1B.
RNA stability at 2–8°C: IL1B.
Blood was drawn from 10 donors, with duplicate samples, and stored at 2–8°C for the indicated number of days, followed by total RNA purification. [A] Blood was collected and stored in PAXgene [...]
RNA stability at 18–25°C: FOS.
RNA stability at 18–25°C: FOS.
Blood was drawn from 10 donors, with duplicate samples, and stored at 18–25°C for the indicated number of days, followed by total RNA purification. [A] Blood was collected and stored in PAXgene [...]
RNA stability at 18–25°C: IL1B.
RNA stability at 18–25°C: IL1B.
Blood was drawn from 10 donors, with duplicate samples, and stored at 18–25°C for the indicated number of days, followed by total RNA purification. [A] Blood was collected and stored in PAXgene [...]
Reproducibility between automated and manual protocols.
Reproducibility between automated and manual protocols.
RNA was purified by 3 different operators (A, B, C) using 3 different lots (1, 2, 3) of the PAXgene Blood RNA Kit using the automated protocol in the experiment described in the figure "RNA yield and [...]
RNA yield and purity — automated processing.
RNA yield and purity — automated processing.
Blood samples from 48 different donors were collected in PAXgene Blood RNA Tubes (6 tubes per donor, 288 tubes in total). The contents of the tubes from 6 donors were pooled and subsequently [...]
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Performance

The patented additive in PAXgene Blood RNA Tubes protects RNA molecules from degradation by RNases and other enzymes, and triggers the complete lysis of all cells in the sample to minimize ex vivo changes in gene expression due to gene induction and downregulation. The result is immediate and long-term stabilization of RNA in specimens, even after being stored for up to 3 days at room temperature (see RNA stability at 18–25°C: IL1B and RNA stability at 18-25°C: FOS) or up to 5 days refrigerated (see RNA stability at 2–8°C: FOS and RNA stability at 2–8°C: IL1B). Currently available data show stabilization of intracellular RNA for up to 8 years at –20°C or –70°C (see In situ stability of RNA in blood specimens stored for 8 years (96 months) at –20°C and –70°C in PAXgene Blood RNA Tubes).

 

Principle

The collection of whole blood is the first step in many molecular assays used to study intracellular RNA. A major challenge in this type of testing is the instability of intracellular RNA, which rapidly degrades within hours after blood collection. Furthermore, certain transcript levels change after blood collection due to ex vivo gene regulation, such as induction and repression. Both RNA degradation and gene regulation can lead to an under- or overestimation of in vivo gene expression profiles.

The PAXgene Blood RNA Tube contains an additive that stabilizes the in vivo gene expression profile by reducing post-collection RNA degradation and minimizing gene induction and repression. When used in conjunction with one of the PAXgene Blood RNA Kits, the PAXgene Blood RNA Tube provides pure RNA for accurate detection and quantification of gene expression.

 

Procedure

Whole blood is collected directly into the PAXgene Blood RNA Tube, using standard phlebotomy practice and equipment. The RNA in the specimen is immediately stabilized for storage at room temperature, refrigerated, or frozen. RNA can be purified using one of the PAXgene Blood RNA Kits (choose the right PAXgene Blood RNA Kit). The ease of collection, stabilization of RNA at different temperatures, and elimination of additional processing steps (e.g., centrifugation, storage on ice) standardizes the workflow from collection to purification, reducing variability that can be introduced by different operators (see Reproducibility between users).

 

Applications

When used in conjunction with the PAXgene Blood RNA Kit, the PAXgene Blood RNA Tubes enable purification of intracellular RNA from whole blood for applications in molecular diagnostic testing, such as real-time RT-PCR analysis of specific transcripts.

 


PAXgene Blood RNA Tubes are intended for in vitro diagnostic testing (IVD) and are to be used in conjunction with the PAXgene Blood RNA Kit.

Handbooks (1)

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PAXgene Blood RNA Tube Product Circular 6688 KB pdf

Brochures (2)

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How to Collect Blood 639 KB pdf
PAXgene Blood RNA System Brochure 811 KB pdf

Technical information (2)

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Typical RNA Yields from PAXgene Blood RNA Tubes processed with the PAXgene Blood RNA Kit 1073 KB pdf
In situ stability of RNA in blood samples stored for 8 years (96 months) at –20°C and –70°C in PAXgene Blood RNA Tubes 2716 KB pdf

Safety Data Sheets (1)

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MSDS PAXgne Blood RNA Tube 121 KB pdf

Posters (5)

File sizeFormatDownload
In Situ Stability of RNA in Blood Samples Stored at –20°C and –70°C in PAXgene Blood RNA Tubes; Guenther et al., ISBER, 2009 83 KB pdf
Automated, Low-Throughput RNA Purification from Whole Blood Using the PAXgene Blood RNA System; Guenther et al., CHI Genomic Sample Prep, 2009 537 KB pdf
Maintaining the Stability and Integrity of RNA from Whole Blood Samples; Guenther and McCluskey, CLI, 2008 3343 KB pdf
Development and Optimization of a Protocol for Automated RNA Purification Using the PAXgene Blood RNA System; Guenther et al., AACR, 2007 2365 KB pdf
Performance Evaluation Study of the PAXgene Blood RNA System with Regulatory Compliance; Guenther et al., AMP, 2005 313 KB pdf

References (2)

File sizeFormatDownload
PAXgene Blood RNA Collection Link
PAXgene Blood RNA Freezing Link

FAQ (13)

    Phlebotomy and Sample Handling with the PAXgene Blood RNA Tube

    1. Why is a blood collection set required to be used with this tube?

    A blood collection set is required to be used with the PAXgene Blood RNA Tube to eliminate the possibility of patient contact with the reagent in the tube, due to backflow of blood, when used in accordance with the instructions for use.

    2. Is there any chance of the reagent going back into the patient's arm?

    If used according to the instructions for use, there is no possibility of backflow during phlebotomy, and thus no possibility of reagent going into the patient’s arm.

    3. Can another company's blood collection set be used with this tube?

    Yes, as long as the phlebotomy instructions included with the PAXgene Blood RNA Tubes are properly followed.

    4. In which order should the PAXgene Blood RNA Tube be drawn, if other tubes are used?

    The PAXgene Blood RNA Tube should always be the last tube drawn.

    5. Why do I have to use a discard tube?

    The discard tube is used to fill the volume of the blood collection set tubing with blood so the subsequent draw into the PAXgene Blood RNA Tube is correct (2.5 ml). If other tubes are being drawn, they can also serve the purpose of a discard tube since the PAXgene Blood RNA Tubes are the last tubes drawn.

    6. Can the PAXgene Blood RNA System be used with animal blood samples?

    The PAXgene Blood RNA System was developed for intracellular RNA isolation from human blood samples. QIAGEN offers RNA stabilization and isolation systems for animal blood. Please contact QIAGEN technical service (www.qiagen.com) for more information.

    7. Can the PAXgene Blood RNA System be used for human body fluids other than blood samples or cultured cells of human origin?

    The PAXgene Blood RNA System was developed for intracellular RNA isolation from human blood specimens. PreAnalytiX offers a dedicated collection and RNA extraction system for human bone marrow (see PAXgene Bone Marrow RNA System). QIAGEN offers RNA stabilization and isolation systems for saliva and cultured cells. Please contact QIAGEN technical service (www.qiagen.com) for more information.

    8. What are possible reasons for blood draws with lower than expected blood volume?

    Possible reasons for blood draws with lower than expected blood volume include: 1) The phlebotomist has not waited enough time for the blood to stop flowing into the tube. 2) The tube has "pushed back" from the needle end attached to the holder and the tube is no longer drawing. 3) Discard tube was not used. For further information see the video Collecting Specimens In PAXgene Blood RNA Tubes and Instructions for Use.

    9. Can blood be pipetted from an EDTA tube into the PAXgene Blood RNA Tube?

    No. Transferring blood from any other tube into the PAXgene Blood RNA Tube is not recommended. The tube and its contents are optimized for performance on blood that is drawn directly into the tube at the recommended draw volume (2.5 ml). The use of anti-coagulated whole blood transferred by pouring or any other method is not recommended.

    10. Can blood be injected from a syringe into the PAXgene Blood RNA Tube?

    No. Using a syringe to collect and transfer blood into the PAXgene Blood RNA Tube is not recommended. This defeats the purpose of immediate stabilization of the sample that the direct-draw tube provides.

    Transport and Storage of the PAXgene Blood RNA Tube

    1. Can the PAXgene Blood RNA Tubes undergo freeze/thaw cycles?

    PAXgene Blood RNA Tubes can go through up to two freeze/thaw cycles with no significant impact on yield or mRNA stability.

    2. How should the PAXgene Blood RNA Tubes be frozen after collection?

    The PAXgene Blood RNA Tubes should be frozen at –20°C and –70°C in a wire rack, not in an expanded polystyrene foam tray. If PAXgene Blood RNA Tubes are to be stored at temperatures lower than –20ºC, it is recommended to freeze them first for 24 h at –20ºC, and then transfer them to –70ºC.

    3. How long can the tubes be kept frozen at –20°C or –70°C?

    Currently we have data for storage at –20°C and –70°C for 96 months (In situ stability of RNA in blood specimens stored for 8 years (96 months) at –20°C and –70°C in PAXgene Blood RNA Tubes). Long term archiving studies are ongoing. See technical note: In Situ Stability of RNA in Blood Samples Stored at –20°C and –70°C in PAXgene Blood RNA Tubes.

ProductCatalog Nr.Price (USD)

PAXgene Blood RNA Tube (100)

100 Blood collection tubes (2.5 ml). To be used in conjunction with PAXgene Blood RNA Kits.
762165 (BD)