We suggest that you are visiting our website from:
United States
Choose your location:
(Please make sure that COOKIES are enabled on your browser)
Go

PAXgene Bone Marrow RNA Tube

For stabilization, transport, and storage of intracellular RNA in bone marrow aspirate samples

  • To be used in conjuction with the PAXgene Bone Marrow RNA Kit
  • Immediate stabilization of intracellular RNA upon introducing the sample into the tube
  • Rapid purification of RNA from heterogeneous bone marrow samples using the
    PAXgene Bone Marrow RNA Kit
  • RNA is stable for days at room temperature or refrigerated
  • Longer-term sample storage at –20°C or –70°C

 

Feature Specification
Tube size 16 x 125 mm
Specimen volume needed 2 ml
Additive/concentration Additive 11.1 ml
Length of stabilization up to 3 days at room temperature (15–25°C)
up to 5 days at 2–8°C
Long-term at –20°C or –70°C
Closure type/color BD Hemogard/Red
Label type Paper
Quantity 50/box

 

Intended use

The PAXgene Bone Marrow RNA System consists of a tube (PAXgene Bone Marrow RNA Tube) containing an RNA stabilizing additive and a nucleic acid preparation kit (PAXgene Bone Marrow RNA Kit). It is intended for the stabilization, transport, and storage of intracellular RNA in a human bone marrow aspirate (BMA) sample and the subsequent isolation and purification of total RNA.

Do not use for blood collection.

For research use only. Not for use in diagnostic procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease. The performance characteristics of this product have not been fully established.

PAXgene Bone Marrow RNA procedure.
stability of 5 transcripts at room temperature.
Stability of 5 transcripts at room temperature.
Bone marrow samples were collected from 39 individuals and immediately transferred and stored in PAXgene Bone Marrow RNA Tubes or EDTA tubes. RNA was purified from the samples on day 0 and day 2 [...]
High RNA integrity after freeze–thaw cycles.
High RNA integrity after freeze–thaw cycles.
Bone marrow samples were stabilized in PAXgene Bone Marrow RNA Tubes, and RNA was purified using the PAXgene Bone Marrow RNA Kit after the indicated number of freeze–thaw cycles. The RIN values did [...]
RNA stability at room temperature.
RNA stability at room temperature.
Bone marrow samples were collected from 39 individuals and immediately transferred to [A] PAXgene Bone Marrow RNA Tubes or [B] EDTA tubes. RNA was purified using the PAXgene Bone Marrow RNA Kit or a [...]
RNA yields from bone marrow samples.
Bone marrow samples were stabilized in PAXgene Bone Marrow RNA Tubes, and RNA was purified using the PAXgene Bone Marrow RNA Kit. Yields are indicated for 295 samples, from both in-house and external [...]
RNA stabilized at 2–8°C.
RNA stabilized at 2–8°C.
Bone marrow samples were collected in duplicate and stabilized in PAXgene Bone Marrow RNA Tubes. RNA was purified from the samples using the PAXgene Bone Marrow RNA Kit with no storage or after 5 [...]
Open lightbox
<
>

Performance

A unique additive in PAXgene Bone Marrow RNA Tubes protects RNA molecules from degradation by RNases and other enzymes, as demonstrated by high RNA integrity numbers (RIN) measured in samples even after 5 days of storage at 2–8°C (see RNA stabilized at 2–8°C). Preservation of RNA integrity is maintained even through freeze-thaw cycles (see High RNA integrity after freeze-thaw cycles). The additive also completely lyses all cells in the samples to halt cellular functions and minimize ex vivo changes in gene expression profiles (see  Stability of 5 transcripts at room temperature).

 

Principle

Copy numbers of individual mRNA species in bone marrow can change significantly during storage or transport at ambient temperature, making reliable studies of gene expression impossible. A unique additive in the PAXgene Bone Marrow RNA Tubes lyses cells, halts cellular processes, and protects RNA from degradation, stabilizing the RNA in the sample for storage at room temperature, refrigerated, or frozen.

The PAXgene Bone Marrow RNA Tubes are optimized for use with the PAXgene Bone Marrow RNA Kit for the efficient isolation and purification of intracellular RNA from heterogeneous human whole bone marrow samples (see  RNA yields from bone marrow samples). RNA can be purified days after collection or the bone marrow sample can be archived for several months at –20°C or –70°C. Intact, high-quality RNA is purified from samples stored at room temperature, refrigerated, or frozen (see RNA stability at room temperatureRNA stabilized at 2–8°C, and High RNA integrity after freeze–thaw cycles).

 

Procedure

Bone marrow aspirates are collected using standard medical practice. A 2 ml sample is then transferred into a PAXgene Bone Marrow RNA Tube (see PAXgene Bone Marrow RNA procedure). The RNA in the sample is immediately stabilized for storage at room temperature, refrigerated, or frozen. RNA can be purified using the PAXgene Bone Marrow RNA Kit.

 

Applications

RNA is stabilized in PAXgene Bone Marrow RNA Tubes and can be used in a range of downstream applications, including:

  • cDNA synthesis
  • Gene expression arrays
  • End-point RT-PCR
  • Quantitative RT-PCR, including TaqMan technology

 

Handbooks (1)

File sizeFormatDownload
PAXgene Bone Marrow RNA Tube product circular - For the collection of human bone marrow and immediate stabilization of intracellular RNA 191 KB pdf

Brochures (1)

File sizeFormatDownload
PAXgene Bone Marrow RNA System Brochure 250 KB pdf

Safety Data Sheets (1)

File sizeFormatDownload
MSDS PAXgene Bone Marrow RNA Tubes Link

Posters (3)

File sizeFormatDownload
Evaluation of the PAXgene Bone Marrow RNA System for Stabilization and Purification of Cellular RNA; Guenther et al., AMP 2006 134 KB pdf
Preanalytical mRNA Stabilization of Whole Bone Marrow Samples; Langebrake et al., Clinical Chemistry, 2007 303 KB pdf
RNA Stabilization of Human Whole Bone Marrow Aspirates for High Reliability of Gene Expression Analysis; Guenther et al., AACR 2007 444 KB pdf

FAQ (8)

    Sample Handling with the PAXgene Bone Marrow RNA Tube

    1. Can the PAXgene Bone Marrow RNA Tube be used for diagnostic or prognostic procedures?

    No. The product is ‘For Research Use.’ Not for use in diagnostic procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.

    2. Can the PAXgene Bone Marrow RNA System be used with animal bone marrow or with blood samples?

    The PAXgene Bone Marrow RNA System was developed for intracellular RNA isolation from human bone marrow samples. Bone marrow samples from other species or from blood were not tested.

    3. Can the PAXgene Bone Marrow RNA System be used for human body fluids other than bone marrow samples or cultured cells of human origin?

    No. The PAXgene Bone Marrow RNA System was developed for intracellular RNA isolation from human bone marrow samples. PreAnalytiX offers a dedicated collection and RNA extraction system for human blood (see the PAXgene Blood RNA System). QIAGEN offers RNA stabilization and isolation systems for saliva and cultured cells. Please contact QIAGEN technical service (www.qiagen.com) for more information.

    4. Can bone marrow aliquots other than 2 ml be pipetted into the PAXgene Bone Marrow RNA Tube?

    No. Transferring bone marrow either larger or smaller than 2 ml into the PAXgene Bone Marrow RNA Tube is not recommended. The tube and its contents were optimized for performance with 2 ml of bone marrow aspirate.

    5. How much time may the aspirated bone marrow sample be allowed to incubate before pipetting it into the PAXgene Bone Marrow RNA Tube?

    Transcript levels begin to change in as few as 10 minutes after a sample is removed from the human body. We highly recommend transferring the sample into the PAXgene Bone Marrow RNA Tube and mixing with the stabilization solution as soon as possible after collection in a syringe.

    Transport and Storage of the PAXgene Bone Marrow RNA Tube

    1. Can bone marrow samples be frozen directly in PAXgene Bone Marrow RNA Tubes at –70°C?

    No. If samples in PAXgene Bone Marrow RNA Tubes are to be stored at temperatures lower than –20ºC, freeze them first for 24 h at –20ºC, then transfer them to –70ºC.

    2. How should the PAXgene Bone Marrow RNA Tubes be frozen after collection?

    Samples in PAXgene Bone Marrow RNA Tubes should be frozen at –20°C and –70°C in a wire rack, not in an expanded polystyrene foam tray. If PAXgene Bone Marrow RNA Tubes are to be stored at temperatures lower than –20ºC, it is recommended to freeze them first for 24 h at –20ºC, and then transfer them to –70ºC.

    3. How long can the tubes be kept frozen at –20°C or –70°C?

    Currently available data shows stabilization of cellular RNA for several months at -20°C or 
    -70°C. For more information from ongoing studies evaluating stability for longer time periods, please contact QIAGEN technical service (www.qiagen.com).

ProductCatalog Nr.Price (USD)

PAXgene Bone Marrow RNA Tubes (50)

50 Bone Marrow Collection Tubes (2 ml). To be used in conjunction with the PAXgene Bone Marrow RNA Kit.
7641141048.00