PAXgene Blood DNA Tube
For collection and stabilization of whole blood samples
- Single tube for collection, stabilization, transport, and storage
- Immediate stabilization of DNA at collection
- Safe and easy transportation and storage at several temperatures
- Standardized preanalytic processing of samples
|Tube size||16 x 100 mm|
|Blood draw volume||8.5 ml|
|Additive/concentration||Additive 2 ml|
|Length of stabilization||up to 14 days at room temperature (15–25°C)
up to 28 days at 2–8°C
up to 3 months at –20°C
Long-term storage at –70°C
|Closure type/color||BD Hemogard/Blue|
The PAXgene Blood DNA Tube is intended for the collection of whole blood for genomic DNA isolation. It is intended to be used as part of the PAXgene Blood DNA System, which additionally includes the PAXgene Blood DNA Kit.
For research use only. Not for use in diagnostic procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease. The performance characteristics of this product have not been fully established.
Multiplex PCR of fragments from the mitochondrial genes tRNAlys/ATPase (0.92 kb), tRNAleu(UUR) (0.63 kb), and ND4 (0.29 kb), using 250 ng DNA from 8 donors as starting material.
Genomic DNA isolated from 8 blood donors using the PAXgene Blood DNA System. [A] Agarose gel analysis; [B] pulsed-field gel electrophoresis for enhanced separation of high-molecular-weight genomic DNA. M: markers.
Blood samples (8.5 ml) are collected in PAXgene Blood DNA Tubes, where they may be stored or transported. For DNA isolation, the blood is transferred to processing tubes (supplied already filled with cell lysis buffer), and the solution is mixed to lyse red and white blood cells. Cell nuclei and mitochondria are pelleted by centrifugation, washed, and resuspended in digestion buffer. Protein contaminants are removed by incubation with a protease. DNA is precipitated in isopropanol, washed in 70% ethanol, dried, and resuspended in resuspension buffer.
The PAXgene Blood DNA Tubes simplify the collection process of whole blood for subsequent DNA purification by enabling collection, immediate stabilization, transportation, and storage of blood samples in the same tube. DNA purified from specimens in PAXgene Blood DNA Tubes using the PAXgene Blood DNA Kit is of high quality and high molecular weight, attesting to the superior stabilization afforded (see High quality and high molecular weight of genomic DNA).
The PAXgene Blood DNA Tubes are closed, evaluated collection tubes containing a proprietary blend of reagents that both prevents blood coagulation and stabilizes genomic DNA in white blood cells. Because this stabilization is afforded at several temperatures, the PAXgene Blood DNA Tubes also serve as vessels for the storage and transportation of blood samples, and stabilization is robust to variation in handling.
Blood samples are collected using standard phlebotomy practice directly into PAXgene Blood DNA Tubes. Samples can then be immediately processed, stored, or shipped within the same tube. Because the PAXgene Blood DNA Tubes are carefully calibrated, the tube draws the 8.5 ml blood needed to maintain the correct additive to blood ratio (0.24) for optimal stabilization.
The DNA purified from whole blood collected and stored in PAXgene Blood DNA Tubes can be used in a wide range of downstream applications, including:
- PCR, multiplex, long-range, and quantitative, real-time PCR
- Southern blotting
- Pharmacogenomic studies
- SNP discovery and SNP genotyping
- Next generation sequencing
|PAXgene Blood DNA Tube (RUO) Product Circular||200 KB|
|Important Note for PAXgene Blood DNA Tube User||73 KB|
|PAXgene Blood DNA Tube Freezing Guidelines||24 KB|
|Reducing Variability and Improving Workflow in the Collecting, Transporting, and Processing of Blood Samples for Genomic DNA Purification Using the PAXgene Blood DNA System; Groelz et al., ASHG, 2004||917 KB|
Sample Collection and Phlebotomy
1. Do I have to use a blood collection set with the PAXgene Blood DNA Tube?
No, a blood collection set does not have to be used with the PAXgene Blood DNA Tube. Further information on phlebotomy technique with the PAXgene Blood DNA Tube can be found in the product circular.
2. Are there any special considerations for phlebotomy when using the PAXgene Blood DNA Tube (blood collection set, discard tube, positioning of the tube, issues with short draws, etc.).
No, blood collection is performed under a standard phlebotomy protocol into the evacuated tube using a needle or blood collection set and a standardized needle holder that can accommodate a 16 mm diameter tube. For more information on phlebotomy technique, please refer to the PAXgene Blood DNA Tube product circular.
3. What is the reagent in the tube?
The PAXgene Blood DNA Tube contains a proprietary EDTA formulation. Contents of the tube may cause irritation to eyes, respiratory system and skin. Please refer to the PAXgene Blood DNA Tube product circular or for additional information, visit the PreAnalytiX website or contact QIAGEN Technical Service to obtain the MSDS sheet.
4. Are the PAXgene Blood DNA Tubes sterile?
The inside of the PAXgene Blood DNA Tube is sterilized during production.
5. What is the shelf life of the PAXgene Blood DNA Tube prior to blood collection?
The expiration date is printed on the tube label and on the shelf carton. Store the PAXgene Blood DNA Tubes at room temperature (18–25°C).
1. How should blood samples drawn into PAXgene Blood DNA Tubes be shipped?
See subsequent FAQs for specific storage temperature and time information. Individual laboratories should validate shipping procedures.
Sample Storage and Archiving
1. How should blood samples drawn into the PAXgene Blood DNA Tubes be stored?
Blood samples can be stored at 15–25°C for up to 14 days or at 2–8°C for up to 28 days. For longer-term storage, freezing the samples at –70ºC or –80°C is recommended.
2. Can the PAXgene Blood DNA Tube be frozen? If yes, at what temperatures and for how long?
The PAXgene Blood DNA Tubes can be stored at –20ºC for up to 3 months. If tubes are to be kept at temperatures below –20ºC, freeze them first at –20ºC for 24 hours, then transfer them to –70ºC or –80ºC.
To freeze PAXgene Blood DNA Tubes, stand them upright in a wire rack or horizontally in a plastic bag. Do not freeze tubes upright in a styrofoam tray as this may cause the tubes to crack.
3. How long can the PAXgene Blood DNA Tube be kept at room temperature? What is room temperature?
The PAXgene Blood DNA Tubes can be kept at room temperature for up to 14 days after blood collection. Room temperature is designated as 18–25°C.
4. Can the PAXgene Blood DNA Tubes be stored at 4°C?
Yes, the PAXgene Blood DNA Tubes can be stored at 4°C for up to 28 days after blood collection.
5. Can the PAXgene Blood DNA Tubes be stored at higher temperatures?
Yes, the PAXgene Blood DNA Tubes can be stored at 30°C for up to 7 days after blood collection. However it is not recommended to store blood sample at temperatures higher than 30°C.
DNA Isolation and Processing
1. How should I thaw frozen PAXgene Blood DNA Tubes?
Thaw the PAXgene Blood DNA Tubes in a wire rack at ambient temperature (18–25ºC) for approximately two hours or at up to 37°C in a water bath for approximately 15 minutes. Carefully invert the thawed PAXgene Blood DNA Tubes 10 times.
2. What is the expected yield from 8.5 ml of blood?
Yield of genomic DNA will vary depending on the cell count from the donor blood sample but average range is from 150 µg to 500 µg.
3. What is the expected purity and size of DNA?
The typical A260/A280 ratio is from 1.7 to 1.9. The DNA molecules are up to 200 kb in size, with fragments of 50–150 kb predominating.
4. Can mitochondrial DNA be isolated with the PAXgene Blood DNA System?
Yes, mitochondrial and genomic DNA are both isolated.
5. Does the PAXgene Blood DNA System contain RNA contamination?
No. DNA purified with the PAXgene Blood DNA System is free of detectable RNA contamination. In contrast to commercial salting out methods, additional RNase digestion steps are not necessary.
6. Can smaller volumes of blood be processed from the PAXgene Blood DNA Tube?
No. The volume of Buffer BG1 (lysis buffer) prefilled in the processing tubes, and all the other buffer volumes are optimized for use with blood from one PAXgene Blood DNA Tube.
7. What does the resuspension buffer, Buffer BG4, contain?
Buffer BG4 consists of 10 mM Tris·Cl, pH 8.5.
8. How should DNA purified using the PAXgene Blood DNA System be stored?
DNA purified using the PAXgene Blood DNA System should be dissolved in Buffer BG4 and stored at 2–8°C. For long-term storage, DNA can be stored at –20°C but multiple freezing and thawing cycles should be avoided.
9. Is it possible to interrupt the protocol?
Yes. After dissolving the nuclei pellet in digestion buffer, Buffer BG3 (step 7), samples can be stored up to 7 days at 2–8°C or for one day at room temperature (18–25°C). The protocol can also be interrupted after adding isopropanol (step 10).
10. Can I use the PAXgene Blood DNA Tube to collect blood from animals?
The system is designed for use only with human blood samples.
11. Can I add bleach to the sample preparation waste?
Do not add bleach or acidic solutions directly to the sample preparation waste. CAUTION: The sample preparation waste contains guanidinium hydrochloride from Buffer BG3, which can form highly reactive compounds when combined with bleach. For more information please consult the appropriate material safety data sheets (MSDS).
1. What downstream applications have been tested with DNA purified using the PAXgene Blood DNA System?
So far, we have shown that DNA purified using the PAXgene Blood DNA System can be used in PCR, including long range and multiplex PCR, restriction digestion and Southern analysis, as well as in various SNP detection procedures including,TaqMan, LightCycler and Masscode technologies.
|Product||Catalog Nr.||Price (USD)|
PAXgene Blood DNA Tube (100)100 PAXgene Blood DNA Tubes (8.5 ml). To be used in conjunction with PAXgene Blood DNA Kit.