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QIAsymphony PAXgene Blood RNA Kit

For automated purification of intracellular RNA (including miRNAs) from whole blood

  • Optimized protocol enables effective copurification of miRNA
  • Prefilled reagent cartridges for safety and ease of use
  • High reproducibility through standardized processing
  • Bar codes enable tracking of samples and reagents

 

Feature Specification
Format Magnetic beads
Technology Magnetic particle technology
Sample amount 2.5 ml
Elution volume 80 µl or 120 µl or 200 µl
Main sample type Whole blood
Time per run 2.5 h for 24 samples
Typical RNA yield 8–16 µg/2.5 ml sample
RNA purity 1.8–2.2 (A260/A280)
Processing Automated: QIAsymphony SP

 

Intended Use

The QIAsymphony PAXgene Blood RNA Kit is intended for the purification of RNA from whole blood collected in PAXgene Blood RNA Tubes and processed on the QIAsymphony SP. The QIAsymphony PAXgene Blood RNA Kit is intended for purification of intracellular RNA, including miRNA, from human whole blood. The QIAsymphony PAXgene Blood RNA Kit is not intended for the isolation of genomic DNA or viral nucleic acids from human whole blood.

For research use only. Not for use in diagnostic procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease. The performance characteristics of this product have not been fully established.

High-quality RNA from different donors.
High-quality RNA from different donors.
Blood was collected from 12 donors into PAXgene Blood RNA Tubes. RNA was purified using the QIAsymphony PAXgene Blood RNA Kit on the QIAsymphony SP. RNA was analyzed with an Agilent 2100 Bioanalyzer. [...]
QIAsymphony PAXgene Blood RNA procedure.
QIAsymphony PAXgene Blood RNA procedure.
[A] QIAsymphony SP instrument. [B] Schematic of the QIAsymphony SP
On-board measures against cross-contamination.
On-board measures against cross-contamination.
Tip guards minimize the risk of sample-to-sample cross-contamination. [A] Pipetting channels. [B] Extractor
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Performance

The fully automated QIAsymphony PAXgene Blood RNA procedure provides RNA yields comparable to the manual PAXgene Blood RNA procedure. Typical yields of RNA isolated from 2.5 ml healthy human whole blood (4.6 x 106 - 1.1 x 107 leukocytes/ml) are ≥3 μg for >95% of the samples processed with all PAXgene Blood RNA Kits. The automated QIAsymphony PAXgene Blood RNA procedure provides high-quality RNA from different donor samples with enrichment of small RNA species (see High-quality RNA from different donors). Since yields are highly donor-dependent, individual yields may vary. The integrated DNase treatment removes trace genomic DNA and on-board measures of the QIAsymphony SP minimize cross-contamination (see On-board measures against cross-contamination).

 

Principle

Copy numbers of individual RNA species in blood can change significantly during storage or transport at ambient temperature, making reliable studies of gene expression difficult. Both stabilization of RNA molecules after collection and an efficient RNA purification protocol are needed to maximize insights into expression profiles of whole blood. Based on proven magnetic particle technology, and when used in combination with PAXgene Blood RNA Tubes, the QIAsymphony PAXgene Blood RNA Kit provides a rapid, automated procedure to isolate intracellular RNA, including miRNA, of high quality and purity.

 

Procedure

The purification procedure starts with a centrifugation step to pellet the contents of each PAXgene Blood RNA Tube. After resuspension of the pellet, the tubes are transferred to the QIAsymphony SP. Loading of the reagent cartridge and plasticware is guided and checked by dedicated software. The QIAsymphony SP then carries out the fully automated RNA purification procedure. An integrated DNase treatment ensures that genomic DNA contamination is minimized (see  QIAsymphony PAXgene Blood RNA procedure). The robust and standardized procedure together with on-board measures of the QIAsymphony SP is designed to minimize the risk of sample-to-sample cross-contamination (see  On-board measures against cross-contamination). After purification, a final heat treatment of the eluate enhances performance in downstream applications. Total processing time is about 2.5 hours for 24 samples, which includes a maximum of 25–30 minutes of hands-on time.

 

Applications

RNA purified using the QIAsymphony PAXgene Blood RNA Kit and the QIAsymphony SP is ready for use in a wide range of downstream applications, including:

  • cDNA synthesis
  • Gene expression arrays
  • End-point RT-PCR
  • Quantitative RT-PCR, including TaqMan technology

 

Handbooks (1)

File sizeFormatDownload
QIAsymphony PAXgene Blood RNA Kit Handbook 347 KB pdf

Supplementary Protocols (1)

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Purification of total RNA including miRNA from human whole blood stabilized in PAXgene Blood RNA Tubes using the QIAsymphony SP and the QIAsymphony PAXgene Blood RNA Kit 500 KB pdf

Brochures (1)

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QIAsymphony PAXgene Blood RNA Kit Brochure 457 KB pdf

Technical information (1)

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Typical total RNA Yields from PAXgene Blood RNA Tubes processed with the QIAsymphony PAXgene Blood RNA Kit 78 KB pdf

Safety Data Sheets (1)

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MSDS QIAsymphony PAXgene Blood RNA Kit (96) Link

Posters (2)

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Evaluation of the QIAsymphony SP Workstation for Magnetic Particle-Based Nucleic Acid Purification from Different Sample Types for Demanding Downstream Applications; Kruhoffer et al., JALA 2010 2822 KB pdf
Automated, Magnetic Bead miRNA Enrichment from Stabilized Whole Blood: Development of an Optimized Protocol and Comparison to Established Procedures by the SPIDIA Consortium; Voss et al., AMP 2010 2078 KB pdf

FAQ (10)

    QIAsymphony PAXgene Blood RNA Kit

    1. Which sample types can be processed using the QIAsymphony PAXgene Blood RNA protocol?

    2.5 ml whole human blood collected in PAXgene Blood RNA Tubes.

    2. Do the samples require pretreatment before loading onto the instrument?

    Yes. Samples have to be incubated at least 2 hours at room temperature (18–22°C). Incubation of the PAXgene Blood RNA Tube overnight may increase yields slightly in some cases. Room temperature storage for up to 3 days has no effect on the RNA profile. During the preparation of the RNA the manual pretreatment consists of a centrifugation and a pellet resuspension step (see the QIAsymphony PAXgene Blood RNA Kit Handbook).

    3. What are the expected yields of RNA from PAXgene Blood RNA Tubes using the QIAsymphony PAXgene Blood RNA Kit?

    RNA yields isolated from 2.5 ml healthy, human, whole blood (4.8 x 106 –1.1 x 107 leukocytes/ml) are specified to ≥3 µg for >95% of the samples processed (see Technical Note QIAsymphony PAXgene Blood RNA System). Since yields are highly donor-dependent, individual yields may vary. Mean yields are in the range of 8–10 µg per tube or higher.

    4. How should the samples be stored before purification of RNA?

    Room temperature storage of the filled PAXgene Blood RNA Tubes for up to 3 days may have no effect on the RNA profile. For longer storage, they should be stored at –20°C or –70°C.

    5. How many samples can be processed with the QIAsymphony PAXgene Blood RNA Kit?

    The kit can be used for 96 RNA preps.

    6. What is the maximum number of batches for the QIAsymphony PAXgene Blood RNA protocol for a single run?

    Due to limited plastic consumables on board the instrument, the number of batches for the QIAsymphony PAXgene Blood RNA protocol is limited to three. Therefore up to 72 samples can be processed in a single run.

    7. What elution formats can be used for the QIAsymphony PAXgene Blood RNA protocol?

    We recommend using Elution Microtubes CL, racked (provided) for elution. Alternatively, 2 ml Sarstedt tubes can be used. Other elution formats will be supported in the future, so please refer to the current labware list.

    8. Why is the final heat denaturation step so important?

    Denaturation of the eluate is essential for maximum efficiency in downstream applications, such as RT-PCR, other amplification reactions, or cDNA synthesis. It is not necessary to denature samples more than once; samples remain denatured after freezing and thawing.

    9. Is it possible to isolate genomic DNA or viral nucleic acids with the QIAsymphony PAXgene Blood RNA protocol?

    The product is not for the isolation of genomic DNA or viral nucleic acids from human whole blood.

    10. Can the QIAsymphony PAXgene Blood RNA system be used for diagnostic or prognostic procedures?

    No. The product is For Research Use Only. Not for use in diagnostic procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease. The performance characteristics of this product have not been fully established.

ProductCatalog Nr.Price (USD)

QIAsymphony PAXgene Blood RNA Kit (96)

For 96 preps: 2 Reagent Cartridges, Enzyme Racks, accessories, and RNase-free buffers. To be used in conjunction with PAXgene Blood RNA Tubes.
762635995.00