The composition, amount, quality and integrity of nucleic acids in whole blood specimens can change dramatically upon collection. For example, enzymatic activity causes degradation of nucleic acids, necrosis and apoptosis lead to the release of genomic DNA from blood cells, and ex vivo gene induction and downregulation can skew transcript profiles.
Bearing in mind the different analytical uses for DNA, RNA and miRNA, and the specific alterations these molecules exhibit in collected blood specimens, PreAnalytiX has designed four systems – the PAXgene Blood DNA System, the PAXgene Blood ccfDNA System, the PAXgene Blood RNA System and the PAXgene Blood miRNA System. In each system workflow, a dedicated device for the collection, transport and storage of whole blood is coupled with an optimized nucleic acid preparation kit for the efficient purification of DNA, circulating, cell-free DNA (ccfDNA), RNA and low molecular weight RNAs.
Learn more about blood by visiting our blood knowledge page.