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Have a look at this interesting paper about the feasibility of Prostate PAXgene Fixation for Molecular Research and Diagnostic Surgical Pathology!

In this study,  fresh frozen, formalin-fixed paraffin-embedded (FFPE), and PAXgene-fixed paraffin-embedded (PFPE) tissue preparation methods were compared in radical prostatectomy prostate tissue from 36 patients. A preliminary test of feasibility of using PFPE tissue in routine prostate surgical pathology diagnostic assessment was performed. In addition to comparing histology, immunohistochemistry, and general measures of DNA and RNA integrity in each fixation method,  functional tests of DNA and RNA quality, including targeted Miseq RNA and DNA sequencing, were done and the methods were implemented to relate DNA and RNA yield and quality.

Results suggested that it is feasible to use PFPE tissue for routine robot-assisted laparoscopic prostatectomy surgical pathology diagnostics and immunohistochemistry, with the benefit of significantly improved DNA and RNA quality and RNA picogram yield per nucleus as compared with FFPE tissue.



Feasibility of Prostate PAXgene Fixation for Molecular Research and Diagnostic Surgical Pathology: Comparison of Matched Fresh Frozen, FFPE, and PFPE Tissue.

Högnäs G, Kivinummi K, Kallio HML, Hieta R, Ruusuvuori P, Koskenalho A, Kesseli J, Tammela TLJ, Riikonen J, Ilvesaro J, Kares S, Hirvikoski PP, Laurila M, Mirtti T, Nykter M, Kujala PM, Visakorpi T, Tolonen T, Bova GS.

Am J Surg Pathol. 2017 Oct 3. doi: 10.1097/PAS.0000000000000961. PMID: 28984675

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