Kit
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Kit Content
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Catalog No.
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PAXgene Container (10)
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For collection, fixation, and stabilization of 10 samples: 10 Prefilled Reagent Containers, containing PAXgene Tissue Fix and PAXgene Tissue Stabilizer
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765112
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Principle
Current tissue fixation methods used in traditional histology are of limited use for molecular analysis. Fixatives that contain formaldehyde cross-link biomolecules and modify nucleic acids and proteins. During tissue fixation, storage, and processing, cross-links lead to degradation of nucleic acids. Since cross-links cannot be removed completely, the resulting chemical modifications can cause inhibition in sensitive downstream applications such as quantitative PCR or RT-PCR. In order to enable both molecular and traditional pathology testing from the same specimen, a method is needed for stabilization of molecular content and preservation of morphology.
PreAnalytiX has developed the PAXgene Tissue System* to meet such needs. The system consists of a tissue collection device (the PAXgene Tissue Container for collection, stabilization, storage, and transportation of human tissue specimens) and kits for purification of total RNA, DNA, or miRNA. PAXgene Tissue Containers provide tissue fixation for histopathology studies and enable purification of high-quality nucleic acids from the same sample for molecular analysis. The fixation and stabilization method preserves tissue morphology (see figure “PAXgene Tissue Histology”) and the integrity of nucleic acids without destructive cross-linking and degradation found in formalin-fixed tissues.
For purification of nucleic acids, the system requires the use of PAXgene Tissue Containers for Tissue collection and stabilization, followed by nucleic acid isolation and purification using one of the PAXgene Tissue Kits for RNA, miRNA, or DNA. Together the containers and kits provide a complete preanalytical solution for collection, fixation, and stabilization of tissue, and purification of high-quality nucleic acid for molecular analysis.
Sample collection and stabilization
PAXgene Tissue Containers are dual-chamber containers prefilled with 2 reagents (see figures “PAXgene Tissue Container 01, -02, -03, -04”). PAXgene Tissue Fix rapidly penetrates and fixes the tissue. After fixation, the tissue is removed from the PAXgene Tissue Fix and transferred to PAXgene Tissue Stabilizer in the second chamber of the same container (see figure “PAXgene Tissue Container Workflow”). When the tissue is stored in PAXgene Tissue Stabilizer, nucleic acids and morphology of the tissue sample are stable for a minimum of 3 and a maximum of 7 days at room temperature or for a minimum of 2 and a maximum of 4 weeks at 2–8°C, depending on the type of tissue. Stabilized samples can be embedded in paraffin for histological studies. Nucleic acids can be isolated from the stabilized samples either before or after embedding in paraffin. See the PAXgene Tissue Kit Handbooks for information about DNA, RNA, or miRNA purification from tissue fixed and stabilized with PAXgene Tissue Container.
Applications
• Conventional pathological staining like H&E, Azan, or PAS
• Immunohistochemical staining
• In situ hybridization
• High-quality nucleic acids from the same sample for molecular analysis (see brochure, technical notes, and posters under “Resources” for examples)
*For Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
