Kit
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Kit Content
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Catalog No.
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PAXgene 96 Blood RNA Kit (4)
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4 PAXgene 96 RNA Plates, 4 PAXgene 96 Filter Plates, Buffers, Proteinase K, RNase-free DNase Sets, AirPore Tape Sheets, Collection Vessels. To be used in conjunction with PAXgene Blood RNA Tubes.
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762331
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A complete system for blood collection and RNA purification
The PAXgene 96 Blood RNA Kit* allows high-throughput isolation of total RNA from up to 96 x 2.5 ml human whole blood. Together with PAXgene Blood RNA Tubes (Müller M.C. et al. [2002] Leukemia 16, 2395; Rainen L. et al. [2002] Clin Chem 48, 1883), it provides a complete preanalytical solution for collection, stabilization, and purification of high-quality RNA.
Fast, easy procedure
The high-throughput procedure is easy and efficient. Processing 96 samples in parallel saves time in your clinical research studies; 96 samples can be processed in 3.5 hours. Lysates are applied to the PAXgene 96 Filter Plate and centrifuged to remove cell debris.
The lysate is then transferred to a PAXgene 96 RNA Plate (see figure “PAXgene 96 Blood RNA Procedure”). Residual DNA is removed through a DNase I digestion. Remaining contaminants are removed in three efficient wash steps before pure RNA is eluted.
Reliable and reproducible purification
The robust PAXgene 96 Blood RNA procedure is designed to minimize the risk of sample-to-sample cross-contamination. Typical yields of RNA isolated from 2.5 ml healthy human whole blood (4.6 x 106 – 1.1 x 107 leucocytes/ml) are ≥3 μg for >95% of the samples.
A wide range of applications
RNA purified using the PAXgene 96 Blood RNA Kit is ready for immediate use in a wide range of downstream research applications, including RT-PCR, cDNA synthesis, quantitative RT-PCR (including TaqMan technology), expression-array and expression-chip analysis, poly A+ RNA selection, and northern analysis.
MSDS:
http://www1.qiagen.com/Support/Msds.aspx?catno=000000000000762331
*The PAXgene 96 Blood RNA Kit is intended for Research Use Only. Not for use in diagnostics procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.
