PAXgene Blood miRNA Kit (50)
For 50 RNA preps: PAXgene Spin Columns, PAXgene Shredder Spin Columns, Processing Tubes, Microcentrifuge Tubes, RNase-Free DNase, RNase-Free Reagents and Buffers. To be used in conjunction with PAXgene Blood RNA Tubes
For purification of total RNA, including microRNA, from whole blood stabilized in PAXgene Blood RNA Tubes
The PAXgene Blood miRNA System* consists of PAXgene Blood RNA Tubes for collection, stabilization, and transport of blood, and the PAXgene Blood miRNA Kit for purification of total RNA, including RNA longer than approximately 18 nucleotides. Purification is carried out using silica-based RNA purification technology in a spin-column format. Purification can be carried out manually, using a microcentrifuge, or automated on the QIAcube®. Used with the tubes, the kit provides a complete preanalytical solution for collection and stabilization through to purification of high-quality total RNA, including miRNA, for molecular analysis.
Benefits of the PAXgene Blood miRNA System include:
• Integrated system for collection, stabilization, and purification
• Effective purification of total RNA, including miRNA
• RNA stabilization for up to 3 days at 18–25°C or 5 days at 2–8°C
Integrated system for collection, stabilization, and purification
Blood samples are collected in PAXgene Blood RNA Tubes, which contain a reagent that lyses blood cells and immediately stabilizes intracellular RNA to preserve the gene expression profile (Müller M.C. et al.  Leukemia 16, 2395; Rainen L. et al.  Clin Chem 48, 1883). RNA stabilization is critical for reliable downstream gene expression analysis. Without stabilization, degradation of RNA and upregulation or downregulation of transcripts and miRNAs occur immediately after blood is drawn. Blood samples collected in PAXgene Blood RNA Tubes can be safely stored or transported at 15–25°C for up to 72 hours and at 2–8°C for up to 5 days without showing any significant RNA degradation or changes in transcript levels.
Total RNA >18 nucleotides (including miRNA) is purified from the stabilized blood samples using well-established PAXgene silica-membrane technology. With the PAXgene Blood miRNA Kit, RNA molecules longer than 18 nucleotides are purified. The purified RNA includes both mRNA and small RNAs such as miRNA (see figure “High yields of small RNA species”). The miRNA species can be analyzed using the QIAGEN® miScript PCR System (see figure “Analysis of miRNA species using sensitive miScript assays”), other real-time RT-PCR systems, or expression analysis assays.
High-quality RNA and miRNA for a wide range of applications
The purified total RNA is ready to use and is ideally suited for any downstream research application, including:
• RT-PCR and real-time RT-PCR
• Differential display
• cDNA synthesis
• Northern, dot, and slot blot analyses
• Primer extension
• Microarray analysis
*For Research Use Only. Not for use in diagnostic procedures. No claim or representation is intended to provide information for the diagnosis, prevention, or treatment of a disease.