The PAXgene Blood DNA System standardizes blood collection and DNA purification from whole blood samples. The integration of sample collection and purification and a single processing tube streamlines workflow and shortens processing time, resulting in cost savings. The PAXgene Blood DNA System is the first step to consistent, reliable results.
Standardization
The PAXgene Blood DNA System is comprised of two integrated components: PAXgene Blood DNA Tubes and the PAXgene Blood DNA Kit. PAXgene Blood DNA Tubes are the first blood collection tubes dedicated for DNA purification. Blood collection and sample processing are integrated into a single standardized system, reducing the risk of sample mix-up and cross-contamination. The DNA purification procedure is streamlined to rapidly achieve reproducible yields of high-quality DNA from each sample.
Blood Collection
Blood samples are drawn directly into PAXgene Blood DNA Tubes via standard phlebotomy technique. These tubes, based on proven BD Vacutainer™ technology, contain a proprietary additive that provides buffering conditions optimized for subsequent cell lysis and DNA purification. Whole blood DNA stored in PAXgene DNA Tubes is stable for 14 days at room temperature or for 28 days at 2–8°C. For long-term storage, freezing samples at –70°C to –80°C is recommended.
DNA Purification
The optimized protocol for DNA purification is a single-tube procedure and uses prefilled processing tubes, eliminating the risk of sample mix-up during purification. The process requires only 1 hour for complete processing of 8 blood samples, which is approximately 25% faster than standard salting-out methods. Use of the PAXgene Blood DNA System results in high yields of pure, high-molecular-weight DNA that performs well in many different downstream applications.
