The PAXgene™ 96 Blood RNA Kit allows high-throughput isolation of total RNA from 2.5ml human
whole blood. Together with PAXgene™ Blood RNA Tubes, it provides a complete preanalytical solution
for collection and stabilization through to purification of high-quality RNA.
The high-throughput procedure is simple, fast and efficient, and processing 96 samples in parallel
provides significant time savings in your clinical research studies.
Lysates are applied to the PAXgene™ 96 Filter Plate and centrifuged to remove cell debris. The
lysate is then applied to a PAXgene™ 96 RNA Plate (see flowchart). Residual DNA is removed through
a DNase I digestion. Remaining contaminants are removed in three efficient wash steps before pure
RNA is eluted.
The robust PAXgene™ 96 Blood RNA procedure is designed to minimize the risk of sample-to-sample
cross contamination. The procedure typically yields between 4 and 20 ug of RNA from 2.5 ml human
whole blood and is highly reproducible.
96 Blood RNA Kit FAQs
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